Complete screening and diagnostic solutions for E. coli testing

E. coli Testing Solutions

 

Monitoring and detection of food borne pathogens is a critical step in maintaining product quality and preventing product and quantify food quality indicator organisms, spoilage organisms, and bacterial foodborne pathogens with a broad choice of methods from traditional, selective, and differential agar media to rapid, real-time PCR assays. Thermo Fisher Scientific offers the broadest selections of solutions to meet your testing needs.

 

  • Plate Count: 3M™ Petrifilm™ facilitates easy accurate and reproducible environmental monitoring for E. coli / Coliform to Australian standard (AS:5013) series or ISO equivalent methods.
  • Standard Culture: The Australian Standard (AS:5013 series) method for isolation, detection and confirmation of E. coli / Coliform contamination of food. Thermo Fisher Sceintific offers a comprehensive selection of dehydrated media, prepared media and associtaed equipement and consumables from industry leading brands including Oxoid™, Remel Wellcolex™ and Thermo Scientific™.
  • Rapid Microbiology: The E. coli / Coliform precis method is a rapid Australian Standard (AS:5013 series) equivalent method for isolation, using selective chromogenic media following for the detection of E. coli / Coliform in food, feed and environmental samples.
  • Molecular Biology: Implementation of qPCR offers the fastest time to result with negative results in less than 24 hours. The use of qPCR for rapid detection and typing of bacterial species including E. coli and E. coli / Coliform can be performed on even the most challenging samples for food and environmental samples using our validated qPCR kits and instrumentation. Thermo Fisher Scientic offers the broadest range of qPCR kits and workflows from industry leading brands including, SureTect™, Applied Biosystems™ and Hygiena™.
  • Automated Culture: The TECTA™ system has been optimised to test environmental water, waste water, wash water, potable water and food samples for presence/absence of E. coli, according to ISO 16649-3 (2016).

 

Comparison Chart - Which method meets my needs?

Selection Criteria

  Plate Count Standard Culture Rapid Culture Molecular Biology Rapid Microbiology
Pre-Enrichment -

x

-

x

-
Selective Enrichment -

x

- - -
Plating

x

x

x

-

-
Detection

x

x

x

x

x

Confirmation

-

x

x

-

x

Viability

x

x

x

-

x

Product QC -

x

x

x

x

Environmental QC

x

x

x

x

x

Strain Identification -

x

x

x

-
Quantitative

x

x

x

-

x

Total Time 27-31 hours 3-4 days 24-48 hours 8-24 hours 2-18 hours
 

 

Plate Count

3M™ Petriflm™ E. coli/Coliform Count Plates

 

3M™ Petrifilm™ E. coli/Coliform Count Plates provide a cost-effective, convenient and reliable method for testing equipment, raw materials, food products and manufacturing environmental samples.

 

The 3M Petrifilm E. coli/Coliform Count Plate is a sample-ready-culture-medium system which contains Violet Red Bile (VRB) nutrients, a cold-water-soluble gelling agent, an indicator of glucuronidase activity (BCIG), and a tetrazolium indicator that facilitates colony enumeration in food and beverage samples.

 

These plates provide both E. coli and total Coliform Count information with confirmed results in just 24-48 hours. By eliminating subsequent confirmation steps required with most traditional reference methods, the 3M Petrifilm E. coli/Coliform Count Plates can help increase productivity and reduce overall lab costs.

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Item Code Description Pack Size  
3M6404 3M Petrifilm TM E.Coli / Coliform Count Plates 50pcs 50 pcs Login to buy
3M6414 3M Petrifilm TM E.Coli / Coliform Count Plates 500pcs 500 pcs Login to buy
 

The 3M™ Petrifilm™ Plate Reader

The 3M™ Petrifilm™ Plate Reader provides consistent, automated reading and recording of results of 3M™ Petrifilm™ Plates (Aerobic, Coliform, E. coli/Coliform Count and Select E. coli) in 4 seconds, thereby increasing productivity, reducing costs and eliminating variation between lab techs. The 3M™ Petrifilm™ Plate Reader reads four 3M™ Petrifilm™ Plates (Aerobic Count, Coliform Count, and E. coli/Coliform Count) in 4 seconds. The included software displays results and color images of the plate on the computer screen, marking the colonies for easy verification. Automatic data storage The 3M Petrifilm Plate Reader automatically exports sample results into a Microsoft Excel spreadsheet. The included 3M software also creates an uneditable log file which provides a historical record of data for audit purposes and enables FDA 21CFR Part 11 compliance. The log file can also be saved in text format to enable easy exporting to a LIMS or other external database.

 

The 3M™ Petrifilm™ Plate Reader provides consistent, automated reading and recording of results of 3M™ Petrifilm™ Plate Reader; Plates (Aerobic, Coliform, E. coli/Coliform Count, Petrifilm Enterobacteriaceae and Select E. coli) in 4 seconds, thereby increasing productivity, reducing costs and eliminating variation between lab techs.

Learn more

 

Item Code Description Pack Size  
3M6499 3M™ Petrifilm™ Plate Reader 1 unit Login to buy

 

Standard Culture

Dehydrated Culture Media

Protect the quality and safety of your finished products with a vast selection of high quality base products, supplements, and special blends, including a wide range of animal-free formulations. All of our dehydrated media is tested for quality and performance before, during, and after manufacturing to make sure our finished product meets the needs of your finished product. All the familiar formulations that you use to ensure your lab performs optimally are right here at your fingertips. Dehydrated Culture Media is available under our legacy brands, Thermo Scientific Oxoid and Thermo Scientific Remel.

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Prepared Culture Media

Whether you are making 100 mL or 100 L, the same steps apply to media preparation. Eliminate many of these, from weighing out and autoclaving, to tempering, pouring or dispensing using ready-prepared media and convenience formats. Let us help you save time and labour.

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Rapid Culture

 

Chromogenic Prepared Media Plates

 

Colour makes a brilliant difference

 

Detect, presumptively identify, and screen samples for pathogenic and non-pathogenic organisms with speed and accuracy using chromogenic media. Colourful, easy-to-read colonies and short incubation times enable timely and important decision-making for both clinical and food testing laboratories.

 

 

E. coli/Coliform plates for food and beverage

Item Code Description Pack Size Format  
PP2609 Brilliance Escherichia coli/Coliform Selective Agar 10 pcs 60 mm Plate Login to buy
PP2313 Brilliance Escherichia coli/Coliform Selective Agar 10 pcs 90 mm Plate Login to buy
TM1686 Brilliance Escherichia coli/Coliform Selective Agar EA 100 mL Login to buy


Learn more about our complete range of Chromogenic Prepared Media Plates

 

SureTect™ qPCR

 

SureTect™ E. coli O157:H7 PCR Assay

Detect E. coli O157:H7 in as little as nine hours with the highly-specific, real-time Thermo Scientific™ SureTect™
E. coli
O157:H7 PCR Assay. The assay incorporates an optimized liquid lysis step, providing a quick and simple method for preparing the sample for rapid detection on the Thermo Scientific™ SureTect™ PikoReal™ or Applied Biosystems™ 7500 Fast Real-Time PCR instruments. The assay has been validated with a variety of matrices, samples sizes, and enrichment volumes, following the AOAC, USDA MLG or NF validated methods, to reduce the need for in-house validation prior to use.


Learn more

 

 
Item Code Description Pack Size  
PT0400A Suretect SureTect™ E. coli O157:H7 PCR Assay 96 tests Login to buy
 

Validated Platforms

QuantStudio™ 5 Real-Time PCR System

The Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use.

  • Proven OptiFlex® technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
  • User account management, locked workflow, and Pause features for greater control of experiment data
  • Built-in software features to support compliance with 21 CFR Part 11 guidelines
  • Secure cloud-based software provides increased accessibility and storage options
  • Simplified instrument software with interactive touchscreen for greater ease of use
  • 96-well 0.2mL format or 96-well 0.1mL format


Learn more

 

 

 

Complete flexibility with Applied Biosystems 7500 Fast System

Expand your in-house PCR capability beyond day-to-day pathogen testing with the Food Safety Applied Biosystems 7500 Fast Real-Time PCR instrument—a versatile, 96-well platform ideal for batch processing several hundred samples a day.



Request more information

 

 

 

RapidFinder™ Analysis Software v1.0

Optimise your pathogen detection workflow and enhance your downstream decision-making process with Thermo Scientific™ RapidFinder™ Analysis Software v1.0. The software simplifies the PCR run setup and the interpretation of pathogen presence/absence results.

 

RapidFinder Analysis Software and the Applied Biosystems™ QuantStudio 5 Food Safety Real-Time PCR System with a 96-well, 0.1-mL block configuration are integral parts of validated workflows for Listeria monocytogenes, Listeria spp., Salmonella spp., Salmonella species, Typhimurium and Enteritidis Multiplex, E. coli O157:H7, and Cronobacter spp. in a variety of food matrices.


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Hygiena PCR

Hygiena BAX® System Q7

To lessen the risk of costly recalls or brand-damaging headlines, food testing labs need fast, accurate and convenient tools for detecting the presence of pathogens in their raw ingredients, environmental samples and finished products. For decades, labs around the world have chosen the award-winning BAX System as their preferred method for food safety testing.

 

With a robust list of both real-time and end-point assays globally validated for a wide variety of foods and environmental sample types, the BAX System Q7 is sure to meet the demands of food processors across the entire supply chain. The Hygiena™ BAX® System offers the accuracy and speed of advanced PCR technology in a format that is safe and simple to use and ideal for routine food testing laboratories.

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BAX® System PCR Assay Kits for E. coli

The BAX® System portfolio of E. coli assays have been designed to meet your needs,
whether you're testing food or environmental samples.

 

 

 

Item Code Description Pack Size  
D14203648 Thermo Scientific BAX System Real-Time E.coli O157 Assay 96 tests 96 tests Login to buy
D14642970 Thermo Scientific BAX System Real-Time STEC Panel 1 Assay 48 tests 48 tests Login to buy
D14642987 Thermo Scientific BAX System Real-Time STEC Panel 2 Assay 48 tests 48 tests Login to buy
 

Complete flexibility with Applied Biosystems 7500 Fast System

Expand your in-house PCR capability beyond day-to-day pathogen testing with the Food Safety Applied Biosystems 7500 Fast Real-Time PCR instrument—a versatile, 96-well platform ideal for batch processing several hundred samples a day.



Request more information

 

 

Other PCR

Applied Biosystems MicroSEQ® Pathogen Detection Kits

Easy-to-use Detection Kits
Applied Biosystems MicroSEQ® Pathogen Detection Kits offer maximum ease of use, reliability, and consistency of results. The reagents in these kits are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC) and other reagents for PCR, and no mixing is required. The kits use specially designed reaction tubes that remain closed throughout the assay process. Once the sample is added, the tubes are closed and remain that way until detection is complete, greatly reducing the chances of contamination.

 

 

 

Detection kits for E. coli/Coliform

RapidFinder™ tests for STEC

The Applied Biosystems™ RapidFinder™ STEC Screening and confirmation assays are part of a complete, validated screening and confirmation workflow enabling TaqMan™ real-time PCR detection of the Shiga toxin-producing E. coli (STEC) O157:H7 and Big 6 non‐O157:H7 serogroup (O26, O45, O103, O111, O121 and O145) in a variety of beef products.

 

Item Code Description Pack Size  
4476901 RapidFinder™ STEC Confirmation Assay 96 tests Login to buy
4476886 RapidFinder™ STEC Screening Assay 96 tests Login to buy

 

MicroSEQ® E. coli

The MicroSEQ® E. coli O157:H7 Detection Kit detects E. coli O157:H7 simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

 

Item Code Description Pack Size  
4445657 MicroSEQ™ E. coli O157:H7 Detection Starter Kit, with PrepSEQ™ Rapid Spin Sample Preparation Kit 1 kit Login to buy
4427409 MicroSEQ™ E. coli O157:H7 Detection Kit 96 tests Login to buy

 

TaqMan® qPCR assays

TaqMan® assay provides a simple, reliable, and rapid procedure for detecting the presence of a specific bacterial or viral target. PCR is used to amplify a DNA target unique to the microorganism, then TaqMan® probe is used to detect and quantify the target in a easy-to-use, single tube or bead format.



View our E. coli range

 

 

Complete flexibility with Applied Biosystems 7500 Fast System

Expand your in-house PCR capability beyond day-to-day pathogen testing with the Food Safety Applied Biosystems 7500 Fast Real-Time PCR instrument—a versatile, 96-well platform ideal for batch processing several hundred samples a day.



Request more information

 
 

Applied Biosystems QuantStudio™ 5 Real-Time PCR System

The Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use.

  • Proven OptiFlex® technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
  • User account management, locked workflow, and Pause features for greater control of experiment data
  • Built-in software features to support compliance with 21 CFR Part 11 guidelines
  • Secure cloud-based software provides increased accessibility and storage options
  • Simplified instrument software with interactive touchscreen for greater ease of use
  • 96-well 0.2mL format or 96-well 0.1mL format


Learn more

 
 

RapidFinder™ Express Software v2.0

RapidFinder™ Express Software v2.0 is a graphical, easy-to-use software for multiple food pathogen detection applications. The software:

 

  • Leads you through the steps to specify the targets of interest, and the number of samples and controls to run.
  • Gives you the flexibility to let the software determine the optimal location for unknown samples and controls on the plate or to customise the plate layout.
  • Leads you through the steps to start an instrument run. The software uses preset, optimised thermal cycling conditions.
  • Automatically generates the results (presence or absence of the pathogen), which you can view for all locations, for each sample, or for individual tubes.


Learn more

 

 

Automated Culture

TECTA™ Rapid Automated Microbial Detection Systems

TECTA™ is an automated microbiological platform that uses patented Polymer Partition technology to rapidly and reliably detect E. coli, Total Coliforms, Faecal Coliforms and Enterococci contamination. Available for testing 4 or 16 samples, this platform is simple, sensitive and flexible, allowing for testing of samples at any time of the day. TECTA™ will automatically transmit data allowing for immediate notification on electronic devices, such as mobile phones or laptops, as soon as a contamination event is detected.

 

TECTA™ is a self-contained ‘lab in a box’ for microbial testing that can be used with just 3 simple steps; add sample, load the cartridge, press start. Two instrument configurations available, the TECTA™ model B4 houses four incubation chambers whilst the TECTA™ model B16 houses sixteen. Each chamber is equipped with a built in spectrometer that is able to actively read and interpret test results. Depending on the level of contamination, positive results can be obtained in as little as two hours and confirmed negative within 18 hours. Both models are network enabled to facilitate notification of results directly to your computer, tablet or smartphone.

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Item Code Description Unit  
ENDTECTAB16AV2-230 TECTA™ B16 V2 Automated microbiology detection 1 system Login to buy
ENDTECTAB4-230 TECTA™ B4 Automated microbiology detection 1 system Login to buy
 

TECTA™ PDS Reagents

The TECTA™ system has been optimised to test environmental water, waste water, wash water, potable water and food samples for presence/absence of E. coli, Total Coliforms, Faecal Coliforms and Enterococci contamination. The TECTA™ ALERT cartridge is Direct-to-Test cartridge, pre-filled with media and reagents (48 tests / box), ready for 100mL of sample.

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Item Code Description Unit  
ENDTECTA-CCA-48 TECTAlert CCA test cartridges for Total Coliforms + E.coli 48/pack Login to buy
ENDTECTA-ECA-48 TECTAlert ECA test cartridges for E.coli (only) 48/pack Login to buy
ENDTECTA-FCA-48 TECTAlert FCA test cartridges for Faecal Coliforms 48/pack Login to buy
ENDTECTA-ENA-48 TECTAlert ENA test cartridges for Enterococci 48/pack Login to buy
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